abstract：:As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...

journal_title：Research in microbiology

authors： Hong HA,Khaneja R,Tam NM,Cazzato A,Tan S,Urdaci M,Brisson A,Gasbarrini A,Barnes I,Cutting SM

更新日期：2009-03-01 00:00:00

abstract：:Six thermophilic extremophiles, Anoxybacillus amylolyticus, Geobacillus thermoleovorans, Geobacillus thermoleovorans subspecies stromboliensis, Geobacillus toebii subspecies decanicus, Bacillus thermantarcticus and Thermus oshimai, isolated from different environmental sites, were studied for their heavy metal resista...

journal_title：Research in microbiology

authors： Poli A,Salerno A,Laezza G,di Donato P,Dumontet S,Nicolaus B

更新日期：2009-03-01 00:00:00

abstract：:Lactobacillus plantarum LA 318 is a potential probiotic strain isolated from normal human intestinal tissue that shows high adhesion to human colonic mucin mediated by the bacterial cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report the adhesion mechanism of the lactobacilli is in part due to GAP...

journal_title：Research in microbiology

authors： Kinoshita H,Wakahara N,Watanabe M,Kawasaki T,Matsuo H,Kawai Y,Kitazawa H,Ohnuma S,Miura K,Horii A,Saito T

更新日期：2008-11-01 00:00:00

abstract：:Cell death induced by adherence of Staphylococcus aureus was observed in Cryptococcus neoformans. To investigate this death mechanism, we examined three factors: actin dynamics using rhodamine-phalloidin staining, accumulation of reactive oxygen species (ROS) via conversion of 2',7'-dichlorofluorescein diacetate to 2'...

journal_title：Research in microbiology

authors： Ikeda R,Sawamura K

更新日期：2008-11-01 00:00:00

abstract：:Bacillus subtilis ATCC6633 produces mycosubtilin, a non-ribosomally synthesized lipopeptide of the iturin family which presents antagonistic activities toward various phytopathogens. Different homologues with fatty acid moiety varying from C15 to C17 are usually co-produced, with their biological activities increasing...

journal_title：Research in microbiology

authors： Fickers P,Leclère V,Guez JS,Béchet M,Coucheney F,Joris B,Jacques P

更新日期：2008-07-01 00:00:00

abstract：:Staphylococcus xylosus is a commensal species commonly found on the skin of mammals, but also currently used as starter culture for meat fermentation. Most strains of this species colonize by forming a biofilm on abiotic surfaces. We show here that the majority of S. xylosus strains also exhibit extensive colony sprea...

journal_title：Research in microbiology

authors： Dordet-Frisoni E,Gaillard-Martinie B,Talon R,Leroy S

更新日期：2008-05-01 00:00:00

abstract：:The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...

journal_title：Research in microbiology

authors： Debruyne L,Samyn E,De Brandt E,Vandenberg O,Heyndrickx M,Vandamme P

更新日期：2008-03-01 00:00:00

abstract：:Mollicute evolution has been marked by significant changes in genome structure and use of their genetic information. These include a reduction in their genome G+C content and the use by most mollicutes of the UGA universal stop codon as tryptophan. More striking is the size reduction in their genome which, for some sp...

journal_title：Research in microbiology

authors： Sirand-Pugnet P,Citti C,Barré A,Blanchard A

更新日期：2007-12-01 00:00:00

abstract：:The human gastric pathogen Helicobacter pylori possesses an enormous genomic plasticity and diversity that facilitates host adaptation. Despite the ancient association with its human host, this epsilon-proteobacterium can cause gastritis, ulcers and gastric cancer. Here we focus on multiple aspects of the genome level...

journal_title：Research in microbiology

authors： Linz B,Schuster SC

更新日期：2007-12-01 00:00:00

abstract：:Triclosan is an antimicrobial agent found in many consumer products. Triclosan inhibits the bacterial fatty acid biosynthetic enzyme, enoyl-ACP reductase (FabI). Decreased susceptibility to triclosan correlates with ciprofloxacin resistance in several bacteria. In these bacteria, resistance to both drugs maps to genes...

journal_title：Research in microbiology

authors： Tkachenko O,Shepard J,Aris VM,Joy A,Bello A,Londono I,Marku J,Soteropoulos P,Peteroy-Kelly MA

更新日期：2007-10-01 00:00:00

abstract：:The presence of viral dsRNA (L and M fractions) in the cryophilic yeast Saccharomyces bayanus var. uvarum is documented here for the first time. Sixty-eight strains of different origins were analyzed. Most of them did not carry dsRNA; the L fraction was found in seven strains, while 11 strains had both L and M fractio...

journal_title：Research in microbiology

authors： Ivannikova YV,Naumova ES,Naumov GI

更新日期：2007-10-01 00:00:00

abstract：:Identification and classification of bacteriophages remains a cumbersome process even with the use of genotypic approaches, due to the lack of genes present in all phages. Restriction fragment length polymorphism analysis (RFLP) of the viral genome is a universal approach, but RFLP fingerprints obtained on agarose gel...

journal_title：Research in microbiology

authors： Merabishvili M,Verhelst R,Glonti T,Chanishvili N,Krylov V,Cuvelier C,Tediashvili M,Vaneechoutte M

更新日期：2007-09-01 00:00:00

abstract：:A common effluent treatment plant (CETP) is a biological wastewater treatment facility that receives wastewater from different industries. The activated biomass in the CETP survives on a wide range of chemicals with no fixed wastewater characteristics. We carried out a diversity analysis of this activated biomass usin...

journal_title：Research in microbiology

authors： Kapley A,De Baere T,Purohit HJ

更新日期：2007-07-01 00:00:00

abstract：:We present evidence that there are at least three Aspergillus nidulans enzymes which catalyze in vitro the reaction of O-acetylserine (OAS) with sulfide forming cysteine. This activity is shared by cysteine synthase (CS) encoded by the cysB gene, homocysteine synthase encoded by cysD and by at least one more enzyme. M...

journal_title：Research in microbiology

authors： Brzywczy J,Natorff R,Sieńko M,Paszewski A

更新日期：2007-06-01 00:00:00

abstract：:Eighty-one strains of Yersinia enterocolitica biovar 1A representing several serotypes isolated from India, France, Germany and the USA were analyzed using ribotyping, 16S-23S rDNA intergenic spacer length polymorphism analysis (PCR-ribotyping) and gyrB restriction fragment length polymorphism. Ribotyping with BglI, N...

journal_title：Research in microbiology

authors： Gulati PS,Virdi JS

更新日期：2007-04-01 00:00:00

abstract：:The marine psychrophilic bacterium Moritella viscosa is the causative agent of winter ulcer in farmed Atlantic salmon and cod. In this study, the growth requirements of the pathogen were established. The effects of changes in salinity and temperature on growth, surface features and proteomic regulation were also inves...

journal_title：Research in microbiology

authors： Tunsjø HS,Paulsen SM,Mikkelsen H,L'abée-Lund TM,Skjerve E,Sørum H

更新日期：2007-04-01 00:00:00

abstract：:Tellurite-containing media are widely used for the screening and isolation of Shiga toxin-producing Escherichia coli (STEC) O157:H7, but tellurite resistance among non-O157 STEC is poorly characterized. Therefore, we investigated 202 STEC strains representing 61 different serotypes from humans, animals or food for the...

journal_title：Research in microbiology

authors： Orth D,Grif K,Dierich MP,Würzner R

更新日期：2007-03-01 00:00:00

abstract：:A highly effective chlorpyrifos-degrading bacterium strain Dsp-2 was isolated from the polluted treatment system of a chlorpyrifos manufacturer. This strain was preliminarily identified as Sphingomonas sp. based on its morphological, physiological and biochemical tests as well as 16S rDNA analysis. It utilized chlorpy...

journal_title：Research in microbiology

authors： Li X,He J,Li S

更新日期：2007-03-01 00:00:00

abstract：:Translation termination in eukaryotes is mediated by two polypeptide chain-release factors, eRF1 and eRF3. eRF1 recognizes stop signals, while eRF3 is a ribosome-dependent and eRF1-dependent GTPase. eRF1 forms a stable complex with eRF3 in vivo and in vitro. In the present study, a variety of truncated forms of Euplot...

journal_title：Research in microbiology

authors： Song L,Chai BF,Wang W,Liang AH

更新日期：2006-11-01 00:00:00

abstract：:We studied exopolysaccharides (EPSs) produced by Halomonas ventosae and Halomonas anticariensis, two novel species of halophilic bacteria. Under optimum environmental and nutritional conditions, H. ventosae strains Al12(T) and Al16 excreted 28.35 mg and 28.95 mg of EPS per 100 ml of culture medium (34.55 and 38.6 mg o...

journal_title：Research in microbiology

authors： Mata JA,Béjar V,Llamas I,Arias S,Bressollier P,Tallon R,Urdaci MC,Quesada E

更新日期：2006-11-01 00:00:00

abstract：:The role of protein-tyrosine kinases in bacterial polymyxin resistance was assessed by both genetic and biochemical approaches. Each of the two genes, wzc and etk, encoding protein-tyrosine kinases in Escherichia coli, was knocked out by using the PCR-based method of one-step inactivation of chromosomal genes, and the...

journal_title：Research in microbiology

authors： Lacour S,Doublet P,Obadia B,Cozzone AJ,Grangeasse C

更新日期：2006-09-01 00:00:00

abstract：:In this study, the use of flow cytometry to analyze phage-mediated killing of Enterobacter aerogenes under varying conditions of temperature and nutrient availability was assessed. Bacteriophage UZ1, specific for an E. aerogenes strain, was applied at a multiplicity of infection (MOI) of 1 and 1000 to a Teflon surface...

journal_title：Research in microbiology

authors： Verthé K,Verstraete W

更新日期：2006-09-01 00:00:00

abstract：:Species of Alicyclobacillus, Bacillus and Thermus genera were selected in order to study the possible presence of the (ADP-ribosyl)ation system. These bacteria are thermophilic, aerobic, and were isolated from different geothermal sources. Both activity and expression of (ADP-ribosyl)ating proteins were tested in cell...

journal_title：Research in microbiology

authors： Faraone-Mennella MR,De Maio A,Petrella A,Romano M,Favaloro P,Gambacorta A,Lama L,Nicolaus B,Farina B

更新日期：2006-07-01 00:00:00

abstract：:The mechanism by which TrwB acts as a DNA transporter in bacterial conjugation is analyzed. Based on a parallelism between TrwB and F(1)-ATPase, TrwB would use the energy derived from ATP hydrolysis to pump DNA through its central channel, in a manner similar to that used by F(1)-ATPase to produce a rotary movement of...

journal_title：Research in microbiology

authors： Cabezon E,de la Cruz F

更新日期：2006-05-01 00:00:00

abstract：:Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...

journal_title：Research in microbiology

authors： Chai BF,Wang W,Liang AH

更新日期：2006-04-01 00:00:00

abstract：:The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...

journal_title：Research in microbiology

authors： Beltramo C,Desroche N,Tourdot-Maréchal R,Grandvalet C,Guzzo J

更新日期：2006-04-01 00:00:00

abstract：:Long polar fimbriae (LPF) are related to type I fimbriae in genetic organization and were first identified in Salmonella enterica serovar Typhimurium. Four lpfA genetic variants designated lpfA(O157/OI-141), lpfA(O157/OI-154), lpfA(O26) and lpfA(O113) have been identified in Shiga toxin-producing Escherichia coli (STE...

journal_title：Research in microbiology

authors： Toma C,Higa N,Iyoda S,Rivas M,Iwanaga M

更新日期：2006-03-01 00:00:00

abstract：:Formation of organized colony morphology is clearly a result of organized, coordinated behavior of cells within a colony, which reflects changes in the cell environment, nutrient availability, inter- and intracolony signaling and others. Under standard conditions, colony morphology is specific to the particular yeast ...

journal_title：Research in microbiology

authors： Vopálenská I,Hůlková M,Janderová B,Palková Z

更新日期：2005-11-01 00:00:00

abstract：:Urease biogenesis was monitored in the lactic acid bacterium Streptococcus thermophilus during the growth cycle using in-gel detection and a phenol-hypochloride assay. Zymogram analysis, performed in a non-denaturing polyacrylamide gel, enabled visualization of a complex profile of bands whose number and intensity wer...

journal_title：Research in microbiology

authors： Mora D,Monnet C,Parini C,Guglielmetti S,Mariani A,Pintus P,Molinari F,Daffonchio D,Manachini PL

更新日期：2005-11-01 00:00:00

abstract：:Genome analyses have revealed that the genomes of non-photosynthetic bacteria including Bacillus subtilis code for proteins similar to the large subunit of RuBisCO (called RuBisCO-like protein (RLP)). This raises a fundamental question as to their functional relationship to photosynthetic RuBisCO. Recently, we identif...

journal_title：Research in microbiology

authors： Ashida H,Danchin A,Yokota A

更新日期：2005-06-01 00:00:00

abstract：:Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...

journal_title：Research in microbiology

authors： Cavaglieri L,Orlando J,Rodríguez MI,Chulze S,Etcheverry M

更新日期：2005-06-01 00:00:00

abstract：:Mycotoxins are toxic secondary metabolites produced by filamentous fungi that have been detected in food commodities, including grapes and wine. A survey was conducted to assess mycotoxin-producing fungi in grapes destined for wine production. The mycotoxigenic capacity of the isolates was tested in culture media. Gra...

journal_title：Research in microbiology

authors： Serra R,Braga A,Venâncio A

更新日期：2005-05-01 00:00:00

abstract：:Three Salmonella strains isolated from a lizard (Gallotia simoni) in the "Isla del Hierro" (Canary Islands, Spain) were serotyped as Salmonella bongori serotype 13,22:z39:-, which has not been described in the Kauffmann-White scheme of Salmonella serovars. In order to shed light on the assignment of those strains to t...

journal_title：Research in microbiology

authors： Herrera-León S,Saco M,Martínez Silvestre A,Silveira L,Echeita A,Usera MA

更新日期：2005-05-01 00:00:00

abstract：:Glucose kinase (Glk) from Streptomyces coelicolor was purified, characterized biochemically and kinetically, and compared to Glk from Streptomyces peucetius var. caesius. In both Streptomyces, 96% of the enzyme activity was detected in the cytosolic fraction. The use of a Glk activity gel showed no isoforms of the enz...

journal_title：Research in microbiology

authors： Imriskova I,Arreguín-Espinosa R,Guzmán S,Rodriguez-Sanoja R,Langley E,Sanchez S

更新日期：2005-04-01 00:00:00

abstract：:A new type of nitrogen-fixing, oxalate-oxidizing Azospirillum sp. was isolated from the roots of Oxalis pes-caprae. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and 16S rRNA sequence comparison. Optimum growth occurred at 30 degrees C, pH 7.5. Growth was observed a...

journal_title：Research in microbiology

authors： Sahin N

更新日期：2005-04-01 00:00:00

abstract：:Sequences of the lacSZ operon of 29 Streptococcus thermophilus strains from different dairy products were determined. Differences in sequence among the strains were detected within LacS more often than in the LacZ gene. The sequences were aligned and compared and it was possible to gather the strains into three groups...

journal_title：Research in microbiology

authors： Ercolini D,Fusco V,Blaiotta G,Coppola S

更新日期：2005-03-01 00:00:00

abstract：:Adhesion to the intestinal mucosa is considered to be one of the main selection criteria of lactic acid bacteria for probiotic use. Adhesive probiotics are, for example, considered to provide better antagonism against pathogenic bacteria when compared to non-adhesive strains. Here a new model is described for studying...

journal_title：Research in microbiology

authors： Vesterlund S,Paltta J,Karp M,Ouwehand AC

更新日期：2005-03-01 00:00:00

abstract：:A hydrogen-producing bacterial strain was newly isolated from a hot spring and identified as Klebsiella oxytoca HP1 by 16S rRNA gene sequence analysis and detection by BioMerieux Vitek. Important parameters, including substrates, starting pH of culture, temperature and oxygen concentration for batch ferment hydrogen p...

journal_title：Research in microbiology

authors： Minnan L,Jinli H,Xiaobin W,Huijuan X,Jinzao C,Chuannan L,Fengzhang Z,Liangshu X

更新日期：2005-01-01 00:00:00

abstract：:An aerotolerant, psychrotolerant anaerobe, anNAG3, was isolated from an acidic forest floor solution (in situ pH of 4.5). Cells of anNAG3 stained Gram-positive did not form spores, and were not motile. Cells were ovoid, approximately 1 microm long and 0.7 microm wide, mostly in pairs, and contained a multi-layered cel...

journal_title：Research in microbiology

authors： Matthies C,Gössner A,Acker G,Schramm A,Drake HL

更新日期：2004-12-01 00:00:00

abstract：:Several studies indicate Actinobacillus (Haemophilus) actinomycetemcomitans and Fusobacterium nucleatum as etiologic agents of periodontal disease. Immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. This study evaluated the antiproliferative activi...

journal_title：Research in microbiology

authors： do Vale CH,de Oliveira Fraga LA,Costa AS,Tavares CA,Martins-Filho OA,de Macedo Farias L,Roque de Carvalho MA

更新日期：2004-11-01 00:00:00